Spectorophotometric

A Spectrophotometric Method for Measuring Serum Trypsin Inhibitor Capacity George M. Homer, Bernard J. Katchman, and Robert E. Zipf The spectrophotometric conventionality of Schwert and Takenaka (11) for the assay of trypsinesterolytic activity hasbeen special for the measurementof the trypsin inhibitor capacityof blood serum. An averagevalue of 0.99 mg. of trypsin inhibited per milliliter of serum (3 S.D. = ±0.30) wasdetermined for 50 control subjects. affected role serum assayvaluesdeterminedwith this methodhavebeenfoundsimilarto thosedetermined with the limited Kunitz method using l131-labeledcaseinas the substratein the normalrange for a pattern of pathologicconditionsand in the empyreal range for abnormalities of the pancreas and generalized cancer conditions. From the standpointsof accuracy, simplicity, and speed this technic is believed to be an improvementover foregoingmethodsfor the measurementof this serum variable. lIHE NEED for additional blood tests for th e diagnosis of pancreatic dysfunction, flux with our own pursual in diagnostic enzyme studies resulted in front work in this area (1, 2). A continued interest in the serum trypsin inhibitor values in cases of malignancy has pass on ruttish the development of a less complex precisely more(prenominal) accurate procedure than that using 1131-laheled casein as the substrate.

precedent methods use for the measurement of this serum variable include the pursuance: the use of jelly as a substrate, by Hussey and Northrop (3) ; a ref ractometric method using casein as a substrate, by G-rob (4); an assay ground on the dissolution of a blood clot, by Clark et al. (5) ; or hemo! static globulin in gravel of blood, by Christensen (6); fibrin tagged with J131as a substrate, by Shulman (7) ; and the spectroFrom the Department of Research, Miami valley hospital, Dayton, Ohio. Presented in part at the 13th Annual clash of the American Association of Clinical Chemists, Aug. 31, 1961, refreshed York. Supported in part by grants from the...If you want to prolong a wide essay, order it on our website: BestEssayCheap.com

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